Lowest Electrophoresis Equipment Price at Hospital Store:
At Hospital Store, we take pride in offering the lowest Electrophoresis Equipment prices, catering to the needs of doctors, hospitals, laboratories and more. We are committed to providing high-quality gel electrophoresis equipment from top brands, including Bio-Rad, Mupid, Seleo, Thermo scientific, and many more. In addition, we back our offerings with a Price Match Guarantee for these trusted brands.
Electrophoresis Equipment Price Range:
The cost of an electrophoresis equipment can vary significantly, depending on the type, brand and the features it offers. Electrophoresis Equipment Price Ranges between INR 15,000 to INR 20 Lakh.
Detailed Electrophoresis Equipment Price List:
For your convenience, we have categorized our electrophoresis equipment prices based on brands. Below, you will find a detailed price list:
|Best Electrophoresis Equipment||Price List|
|Cell, Giant HS, Fully Auto Electrophoresis Machine||Upto INR 18 Lakh|
|Cell Minilite Plus Automated Electrophoresis System||Upto INR 12 Lakh|
*Please note that prices are subject to change and may vary based on additional features and specifications.
What is electrophoresis equipment?
Electrophoresis equipment is a lab equipment widely utilized for separating macromolecules like DNA, RNA or Protein based on their size and charge. It is based on the electrophoresis principle, which involves applying an electric field on a gel or other media and allowing charged particles to flow through it at varying speeds based on their size and charge.
What are the components of the electrophoresis device?
Here’s an overview of key components of electrophoresis equipment:
- Electrophoresis power supply: Power supply in electrophoresis is responsible to provide the electric field required for initiating the procedure. It let user adjust the voltage and current as per the requirement.
- Gel electrophoresis system: It involves gel itself. It is composed of polyacrylamide or agarose, and a casting device to produce the gel. The molecules will move through the gel as a medium. For DNA separations, agarose gels are commonly utilized, while polyacrylamide gels are the preferred choice for protein separations.
- Sample wells: Wells are made using comb or other tools before it solidifies. These wells are used to load the sample in it.
- Loading buffer: A solution where the sample (such as protein or DNA) is mixed before being added to the wells. Tracking dyes may be added to loading buffer to keep track of the electrophoresis's progress.
- Electrodes: Two types of electrodes are present, the anode (positive) and the cathode (negative) which are connected to the power supply. They are located at either end of the gel. The cathode attracts the positive charged electrodes while the anode attracts the negative molecules.
- Buffer solution: The gel is immersed in a buffer solution. It is responsible to maintain a stable pH and ionic environment to deliver precise result.
- Electrophoresis chamber: An electrophoresis procedure is conducted in an enclosed space with a constant temperature inside a chamber that holds the gel and the buffer.
- Visualization system: Once electrophoresis is finished, you'll want a method of identifying the molecules that have been separated. Many methods, including Coomassie staining for protein gels and UV light for DNA gels, can be used to perform this.
- Documentation system: To record and save the results, a simple camera with a UV transilluminator for gels are used.
What are the different types of electrophoresis techniques?
There are various types of electrophoresis techniques which are utilized in laboratories depending on the type of sample, charge and its size. Each electrophoresis instrument has its own unique features and way of separating macromolecules.
- Agarose gel electrophoresis equipment: This electrophoresis device uses a technique typically intended for the separation of DNA molecules. DNA samples are put into an agarose gel, a porous matrix, and subsequently organized according to size. Larger DNA fragments flow through the gel more slowly than smaller ones.
- Polyacrylamide Gel Electrophoresis (PAGE): This technique offers high resolution. It is typically used to separate small molecules like protein and nucleic acid. A specific type of PAGE called SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) is used to separate proteins according to their molecular weight.
- Capillary electrophoresis instrument: It involves electrophoresis method in a capillary tube consisting of electrolyte buffer. This technique is highly efficient and majorly utilized for DNA sequencing, separation of protein and other small ions & molecules.
- Two- Dimensional Gel electrophoresis (2D- GE): Higher resolution is achieved by combining two well-defined electrophoresis processes in this approach. Proteins are separated in two dimensions: first, according to charge (typically by isoelectric focusing), and second, according to size (SDS-PAGE). In proteomics, 2D-GE is frequently used to analyze intricate protein combinations.
- Pulsed-Field Gel Electrophoresis (PFGE): In this technique, separation of large DNA fragments are initiated such as whole chromosomes.
Differentiate between horizontal electrophoresis system and vertical electrophoresis system.
Electrophoresis procedure can be done in two different orientations, horizontal electrophoresis and vertical electrophoresis. The major difference between them is the direction in which electrophoresis occurs.
1. Horizontal electrophoresis apparatus: In this method, separation of molecules are done in a horizontal axis. Here are the key features of horizontal electrophoresis:
- Samples are usually put into wells that run horizontally across the gel, and the gel is normally cast inside a horizontal gel tray.
- DNA molecules of different sizes are frequently separated using agarose gel electrophoresis.
- PAGE stands for polyacrylamide gel electrophoresis. In SDS-PAGE, proteins often separate with a horizontal orientation.
2. Vertical electrophoresis apparatus: In this orientation, separation occurs in a vertical plane. The key features of vertical electrophoresis are as follows:
- Samples are placed into wells at the top of the gel after it has been poured and positioned vertically, allowing separation to happen vertically as the molecules move downhill.
- Western blotting and native PAGE are two common protein separation techniques that employ vertical electrophoresis.
- It is also applicable in applications such as pulsed-field gel electrophoresis (PFGE) for the electrophoresis of DNA and RNA.